Review

rabbit polyclonal anti dvl1 (Proteintech)

Proteintech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Proteintech rabbit polyclonal anti dvl1
Rabbit Polyclonal Anti Dvl1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+dvl1/pmc12513720-4-2-6?v=Proteintech
Average 93 stars, based on 21 article reviews

rabbit polyclonal anti dvl1 - by Bioz Stars, 2026-08

93/100 stars

Images

Image Search Results

Neuroglobin (Ngb) interacts with Dvl1 in SKNSH cells. ( A ) HA-Ngb was co-transfected with Myc-Dvl1 in SK-N-SH cells. Cells were harvested after 24 h post-transfection. Cells extract (600 μg) were precipitated by anti-Myc rabbit polyclonal antibody or control Immunoglobulin G (IgG). Western blot was used to detect Myc-Dvl1 and HA-Ngb. ( B ) Cells extract (600 μg) were prepared and immunoprecipitation (IP) with rabbit anti-Dvl1 antibody or control rabbit IgG. Western blot was used to detect Dvl1 and Ngb. ( C ) Dvl1(1-250), Dvl1(1-378), Dvl1(337-670) or Myc-Dvl1 was co-transfected with HA-Ngb. After 24 h post-transfection, cells were harvested and IP with mouse anti-Myc monoclonal antibody. Western blot was used to detect Ngb. ( D ) SK-N-SH cells were seeded in 6-well plate. The mouse anti-Ngb monoclonal antibody and Texas Red-conjugated anti-mouse IgG (red) were used to detect Ngb protein. The rabbit anti-Dvl1 monoclonal antibody and Texas Green-conjugated anti-rabbit IgG were used to detect Dvl1 protein. Nuclei were stained by Hoechst 33258. The merged image showed the co-localization of Dvl1 and Ngb. ( E ) SK-N-SH cells were co-transfected with Myc-Dvl1 and HA-Ngb. The rabbit anti-HA polyclonal antibody and Texas Green-conjugated anti-rabbit IgG were used to detect HA-Ngb protein. The mouse anti-Myc monoclonal antibody and Texas Red-conjugated anti-rabbit IgG were used to detect Myc-Dvl1 protein. Nuclei were stained by Hoechst 33258. The merged image showed the co-localization of Myc-Dvl1 and HA-Ngb.

Journal: International Journal of Molecular Sciences

Article Title: Neuroglobin Regulates Wnt/β-Catenin and NFκB Signaling Pathway through Dvl1

doi: 10.3390/ijms19072133

Figure Lengend Snippet: Neuroglobin (Ngb) interacts with Dvl1 in SKNSH cells. ( A ) HA-Ngb was co-transfected with Myc-Dvl1 in SK-N-SH cells. Cells were harvested after 24 h post-transfection. Cells extract (600 μg) were precipitated by anti-Myc rabbit polyclonal antibody or control Immunoglobulin G (IgG). Western blot was used to detect Myc-Dvl1 and HA-Ngb. ( B ) Cells extract (600 μg) were prepared and immunoprecipitation (IP) with rabbit anti-Dvl1 antibody or control rabbit IgG. Western blot was used to detect Dvl1 and Ngb. ( C ) Dvl1(1-250), Dvl1(1-378), Dvl1(337-670) or Myc-Dvl1 was co-transfected with HA-Ngb. After 24 h post-transfection, cells were harvested and IP with mouse anti-Myc monoclonal antibody. Western blot was used to detect Ngb. ( D ) SK-N-SH cells were seeded in 6-well plate. The mouse anti-Ngb monoclonal antibody and Texas Red-conjugated anti-mouse IgG (red) were used to detect Ngb protein. The rabbit anti-Dvl1 monoclonal antibody and Texas Green-conjugated anti-rabbit IgG were used to detect Dvl1 protein. Nuclei were stained by Hoechst 33258. The merged image showed the co-localization of Dvl1 and Ngb. ( E ) SK-N-SH cells were co-transfected with Myc-Dvl1 and HA-Ngb. The rabbit anti-HA polyclonal antibody and Texas Green-conjugated anti-rabbit IgG were used to detect HA-Ngb protein. The mouse anti-Myc monoclonal antibody and Texas Red-conjugated anti-rabbit IgG were used to detect Myc-Dvl1 protein. Nuclei were stained by Hoechst 33258. The merged image showed the co-localization of Myc-Dvl1 and HA-Ngb.

Article Snippet: Cell lysates were incubated with anti-Dvl1 rabbit polyclonal antibodies and Protein A/G plus-agarose (Santa Cruz) at 4 °C overnight.

Techniques: Transfection, Western Blot, Immunoprecipitation, Staining

Ngb promotes the proteasomal degradation of Dvl1. ( A ) SK-N-SH cells were seeded in 6-well plate and transfected with plasmids as described. Cells were harvested after 24 h post-transfection. ( B ) SK-N-SH cells were cultured in 6 cm plate and transfected with the increased amount of HA-Ngb as described. Western blot was used to detect the expression of Dvl1 and Dvl2. densitometric analysis of western blot showed the effect of Ngb on Dvl1 and Dvl2 protein levels. β-actin served as equal loading controls. (Mean ± SD). n = 3, * p < 0.05, ** p < 0.01 versus cells transfected with empty plasmid. ( C ) 2 μg HA-Ngb or empty plasmid (pCMV-HA) was co-transfected with 0.5 μg Myc-Dvl1. After 12 h of post-transfection, cells were treated with CHX for 0, 1, 2, and 4 h. ( D ) Cells were treated with DMSO, MG132, or NH 4 Cl for 8 h. Densitometric analysis of western blot showed the effect of MG132 and NH 4 Cl on the Ngb-induced degradation of Dvl1β-actin served as equal loading controls. (Mean ± SD). n = 3, * p < 0.05, ** p < 0.01 versus cells transfected with controls as described. ( E ) His-Ub and Myc-Dvl1 were co-transfected with HA-Ngb or empty plasmid (pCMV-HA) into SK-N-SH cells. Then the His-ubiquitinated proteins were isolated from cell extracts using NTA agarose. Western blot was used to detect ubiquitinated Dvl1.

Journal: International Journal of Molecular Sciences

Article Title: Neuroglobin Regulates Wnt/β-Catenin and NFκB Signaling Pathway through Dvl1

doi: 10.3390/ijms19072133

Figure Lengend Snippet: Ngb promotes the proteasomal degradation of Dvl1. ( A ) SK-N-SH cells were seeded in 6-well plate and transfected with plasmids as described. Cells were harvested after 24 h post-transfection. ( B ) SK-N-SH cells were cultured in 6 cm plate and transfected with the increased amount of HA-Ngb as described. Western blot was used to detect the expression of Dvl1 and Dvl2. densitometric analysis of western blot showed the effect of Ngb on Dvl1 and Dvl2 protein levels. β-actin served as equal loading controls. (Mean ± SD). n = 3, * p < 0.05, ** p < 0.01 versus cells transfected with empty plasmid. ( C ) 2 μg HA-Ngb or empty plasmid (pCMV-HA) was co-transfected with 0.5 μg Myc-Dvl1. After 12 h of post-transfection, cells were treated with CHX for 0, 1, 2, and 4 h. ( D ) Cells were treated with DMSO, MG132, or NH 4 Cl for 8 h. Densitometric analysis of western blot showed the effect of MG132 and NH 4 Cl on the Ngb-induced degradation of Dvl1β-actin served as equal loading controls. (Mean ± SD). n = 3, * p < 0.05, ** p < 0.01 versus cells transfected with controls as described. ( E ) His-Ub and Myc-Dvl1 were co-transfected with HA-Ngb or empty plasmid (pCMV-HA) into SK-N-SH cells. Then the His-ubiquitinated proteins were isolated from cell extracts using NTA agarose. Western blot was used to detect ubiquitinated Dvl1.

Article Snippet: Cell lysates were incubated with anti-Dvl1 rabbit polyclonal antibodies and Protein A/G plus-agarose (Santa Cruz) at 4 °C overnight.

Techniques: Transfection, Cell Culture, Western Blot, Expressing, Plasmid Preparation, Isolation

Ngb inhibits Wnt signaling pathway through decreasing Dvl1. ( A ) SK-N-SH cells were seeded on 24-well plates. Increased amount of HA-Ngb was co-transfected with constant amount of PRL-TK and pTOPFLASH. Luciferase activity (Mean ± SD) was measured after 24 h post-transfection. n = 3, ** p < 0.01 compared with controls. ( B ) Increased amount of HA-Ngb was transfected into SK-N-SH cells. The level of β-catenin proteins was detected by western blot. Densitometric analysis of western blot was performed. β-actin served as equal loading controls. (Mean ± SD). n = 3, ** p < 0.01 versus cells transfected with empty plasmid. ( C ) HA-Ngb or empty plasmid (pCMV-HA) was co-transfected with Myc-Dvl1 or empty plasmid (pCMV-Myc). Densitometric analysis of western bolt was performed. β-actin served as equal loading controls. (Mean ± SD). n = 3, * p < 0.05, ** p < 0.01 versus controls as described. ( D ) HA-Ngb or empty plasmid (pCMV-HA) was co-transfected with NC siRNA or Dvl1 siRNA. Western blot was performed after transfection for 48 h. Densitometric analysis of western bolt was performed. β-actin served as equal loading controls. (Mean ± SD). n = 3, ** p < 0.01 versus controls.

Journal: International Journal of Molecular Sciences

Article Title: Neuroglobin Regulates Wnt/β-Catenin and NFκB Signaling Pathway through Dvl1

doi: 10.3390/ijms19072133

Figure Lengend Snippet: Ngb inhibits Wnt signaling pathway through decreasing Dvl1. ( A ) SK-N-SH cells were seeded on 24-well plates. Increased amount of HA-Ngb was co-transfected with constant amount of PRL-TK and pTOPFLASH. Luciferase activity (Mean ± SD) was measured after 24 h post-transfection. n = 3, ** p < 0.01 compared with controls. ( B ) Increased amount of HA-Ngb was transfected into SK-N-SH cells. The level of β-catenin proteins was detected by western blot. Densitometric analysis of western blot was performed. β-actin served as equal loading controls. (Mean ± SD). n = 3, ** p < 0.01 versus cells transfected with empty plasmid. ( C ) HA-Ngb or empty plasmid (pCMV-HA) was co-transfected with Myc-Dvl1 or empty plasmid (pCMV-Myc). Densitometric analysis of western bolt was performed. β-actin served as equal loading controls. (Mean ± SD). n = 3, * p < 0.05, ** p < 0.01 versus controls as described. ( D ) HA-Ngb or empty plasmid (pCMV-HA) was co-transfected with NC siRNA or Dvl1 siRNA. Western blot was performed after transfection for 48 h. Densitometric analysis of western bolt was performed. β-actin served as equal loading controls. (Mean ± SD). n = 3, ** p < 0.01 versus controls.

Article Snippet: Cell lysates were incubated with anti-Dvl1 rabbit polyclonal antibodies and Protein A/G plus-agarose (Santa Cruz) at 4 °C overnight.

Techniques: Transfection, Luciferase, Activity Assay, Western Blot, Plasmid Preparation

Ngb enhances TNF-α-induced-NFκB activation via down-regulating Dvl1. ( A ) SK-N-SH cells were treated with 10 ng/mL TNF-α or PBS for 6, 12, or 24 h. ( B ) The plasmids as described were co-transfected with NFκB report plasmid and PRL-TK plasmid in SK-N-SH cells. After transfection for 24 h, cells were treated with 10 ng/mL TNF-α or PBS for 6 h before Luciferase assays were performed. Data were presented as relative luciferase activities normalized to Renilla luciferase activities n = 4, * p < 0.05; ** p < 0.01 versus controls. ( C ) Cells were seeded in 10 cm plates and treated with 10 ng/mL TNF-α or PBS for 1 h. Cells’ lysates were immunoprecipitated with the anti-Dvl1 antibody. Western blot was used to detect Ngb and Dvl1. ( D ) HA-Ngb or empty plasmid (pCMV-HA) was transfected into SK-N-SH cells. After 24 h of post-transfection, cells were treated with TNF-α or PBS for 6 h. ( E ) U6-siNgb or empty plasmid (pRNAT-U6.1) was transfected into SK-N-SH cells. After 24 h of post-transfection, cells were treated with TNF-α or PBS for 6 h.

Journal: International Journal of Molecular Sciences

Article Title: Neuroglobin Regulates Wnt/β-Catenin and NFκB Signaling Pathway through Dvl1

doi: 10.3390/ijms19072133

Figure Lengend Snippet: Ngb enhances TNF-α-induced-NFκB activation via down-regulating Dvl1. ( A ) SK-N-SH cells were treated with 10 ng/mL TNF-α or PBS for 6, 12, or 24 h. ( B ) The plasmids as described were co-transfected with NFκB report plasmid and PRL-TK plasmid in SK-N-SH cells. After transfection for 24 h, cells were treated with 10 ng/mL TNF-α or PBS for 6 h before Luciferase assays were performed. Data were presented as relative luciferase activities normalized to Renilla luciferase activities n = 4, * p < 0.05; ** p < 0.01 versus controls. ( C ) Cells were seeded in 10 cm plates and treated with 10 ng/mL TNF-α or PBS for 1 h. Cells’ lysates were immunoprecipitated with the anti-Dvl1 antibody. Western blot was used to detect Ngb and Dvl1. ( D ) HA-Ngb or empty plasmid (pCMV-HA) was transfected into SK-N-SH cells. After 24 h of post-transfection, cells were treated with TNF-α or PBS for 6 h. ( E ) U6-siNgb or empty plasmid (pRNAT-U6.1) was transfected into SK-N-SH cells. After 24 h of post-transfection, cells were treated with TNF-α or PBS for 6 h.

Article Snippet: Cell lysates were incubated with anti-Dvl1 rabbit polyclonal antibodies and Protein A/G plus-agarose (Santa Cruz) at 4 °C overnight.

Techniques: Activation Assay, Transfection, Plasmid Preparation, Luciferase, Immunoprecipitation, Western Blot

Ngb protects SK-N-SH cells from TNF-α-induced cell death. ( A ) SK-N-SH cells were transfected with empty plasmid (pRNAT-U6.1) or U6-siNgb. After 24 h post-transfection, cells were treated with PBS, 10 ng/mL or 30 ng/mL TNF-α for 24 h. 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was used to measure cells’ viability. (Mean ± SD). n = 3, ** p < 0.01 versus controls. ( B ) Empty plasmid (pCMV-HA), HA-Ngb or HA-Ngb combined with Myc-Dvl1 was transfected into SK-N-SH cells. After 24 h of post-transfection, cells were treated with 30 ng/mL TNF-α for 24 h. Then MTT assay was used to measure cells viability. (Mean ± SD). n = 3, ** p < 0.01 versus controls.

Journal: International Journal of Molecular Sciences

Article Title: Neuroglobin Regulates Wnt/β-Catenin and NFκB Signaling Pathway through Dvl1

doi: 10.3390/ijms19072133

Figure Lengend Snippet: Ngb protects SK-N-SH cells from TNF-α-induced cell death. ( A ) SK-N-SH cells were transfected with empty plasmid (pRNAT-U6.1) or U6-siNgb. After 24 h post-transfection, cells were treated with PBS, 10 ng/mL or 30 ng/mL TNF-α for 24 h. 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was used to measure cells’ viability. (Mean ± SD). n = 3, ** p < 0.01 versus controls. ( B ) Empty plasmid (pCMV-HA), HA-Ngb or HA-Ngb combined with Myc-Dvl1 was transfected into SK-N-SH cells. After 24 h of post-transfection, cells were treated with 30 ng/mL TNF-α for 24 h. Then MTT assay was used to measure cells viability. (Mean ± SD). n = 3, ** p < 0.01 versus controls.

Article Snippet: Cell lysates were incubated with anti-Dvl1 rabbit polyclonal antibodies and Protein A/G plus-agarose (Santa Cruz) at 4 °C overnight.

Techniques: Transfection, Plasmid Preparation, MTT Assay

Journal: International Journal of Molecular Sciences

Article Title: Neuroglobin Regulates Wnt/β-Catenin and NFκB Signaling Pathway through Dvl1

doi: 10.3390/ijms19072133

Figure Lengend Snippet: Primers used in the present study.

Article Snippet: Cell lysates were incubated with anti-Dvl1 rabbit polyclonal antibodies and Protein A/G plus-agarose (Santa Cruz) at 4 °C overnight.

Techniques: Sequencing

Review

Structured Review

Images

Similar Products

Image Search Results